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KMID : 0644120190320040001
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Journal of Oriental Gynecology
2019 Volume.32 No. 4 p.1 ~ p.13
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Antimetastatic Effects of Crude polysaccharide isolated from Polygonati Rhizoma on 4T1 breast cancer cells by Activation of Innate Immune System
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Ji Hae-Ri
Hwang Deok-Sang
Lee Chang-Hoon
Jang Jun-Bock
Lee Jin-Moo
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Abstract
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Objective: This study is aimed to investigate the anti-tumor metastasis by innate immunomodulating effects of crude polysaccharide isolated from Polygonati Rhizoma (CP-PR) on 4T1 breast cancer cells.
Methods: CP-PR was isolated from Polygonati Rhizoma. Antimetastatic experiments were conducted in vivo mouse model by using 4T1 breast cancer cells. The cell viability of CP-PR was tested with normal spleen and 4T1 breast cancer cells. To observe the activation of macrophages with/without 4T1 breast cancer cells, production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10 and IL-12 were measured with ELISA assay, respectively. In addition, the lysis of YAC-1 cells and the production of granzymes were measured to observe the activation of natural killer (NK) cell.
Results: Intravenous administration of CP-PR significantly inhibited metastasis of 4T1 breast cancer cells. In an in vitro cytotoxicity analysis, CP-PR affected the growth of normal spleen and 4T1 breast cancer cells above specific concentration. The production of TNF-α, IL-6, IL-10 and IL-12 were significantly increased in macrophages with CP-PR. As compared with control, CP-PR showed significantly higher production of TNF-α, IL-10 and IL-12 in macrophages co-cultured with 4T1 breast cancer cells. The lysis of YAC-1 cells and the production of granzymes were significantly up regulated by CP-PR.
Conclusion: CP-PR appears to have considerable activity on the anti-metastasis by activation of innate immune system.
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KEYWORD
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Polygonati Rhizoma, polysaccharide, breast cancer, anti-tumor metastasis, innate immune system
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